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Spectrophotometry measures light absorption: A = log(I₀/I) = εbc (Beer-Lambert law), used for quantitative analysis.
Trinn-for-trinn guide
- 1Input measured absorbance, path length, molar absorptivity
- 2Calculate concentration
- 3Generate calibration curve if multiple samples
Løste eksempler
Inndata
Absorbance 0.5, ε = 5000 M⁻¹cm⁻¹, b = 1 cm
Resultat
c = A/(εb) = 0.5/5000 = 1×10⁻⁴ M
Vanlige feil å unngå
- ✕Using wrong wavelength for compound
- ✕Not accounting for baseline absorbance
Ofte stilte spørsmål
What's molar absorptivity?
Characteristic of molecule at specific wavelength; tabulated values for known compounds.
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